Reação do tecido subcutâneo de rato à implantação do MTA branco e Ultrapex com e sem própolis / Reaction of connective tissues rats to implantation of white MTA and Ultrapex with or without propolis

O objetivo deste estudo foi comparar a reação do tecido subcutâneo de rato, frente a implantação de MTA branco-Angelus® e Ultrapex® com e sem própolis. Tubos de polietileno, preenchidos com MTA branco, MTA branco+própolis, Ultrap ex® e Ultrapex®+própolis foram implantados em tecido subcutâneo de 21 ratos Wistar (Rattus norvegicus). Os animais foram mortos aos 30, 45 e 60 dias. O tecido contendo o implante foi removido e processado histologicamente.Cortes seriados de 5μm foram corados pela hematoxilina e eosina em que se avaliou a intensidade do infiltrado inflamatório, característica da cápsula fibrosa e dispersão do material. A esses dados foi aplicado o teste do Qui-quadrado o qual se observou diferença estatisticamente significante entre os quatro materiais experimentais. O MTA branco+própolis foi o que apresentou uma menor reação tecidual, seguido do MTA branco, Ultrapex®+própolis e Ultrapex®. A adição da própolis melhorou o comportamento biológico dos materiais em teste

The aims of this study was to compare the reaction of white MTA-Angelus® and Ultrapex® with and without propolis. Polyethylene tubes filled with white MTA, white MTA+propolis, Ultrapex, Ultrapex®+propolis were implanted in subcutaneous tissue of 21 Wistar rats (Rattus norvegicus). The animals were killed at 30, 45 and 60 days. The tissue containing the implant was removed and processed histologically. Serial sections of 5μm were stained with hematoxylin and eosin. They were evaluated the inflammatory infiltrate, and characteristic of fibrous capsule anddispersal of the material. The data was applied to the Chi-square where there was a statistically significant difference among the four experimental materials. White MTA + propolis showed a more favorable tissue reaction, followed by white MTA, Ultrapex® + propolis and Ultrapex®. The propolis has improved the biological behavior of materials under test

Disseminated malignant fibrous histiocytoma (giant cell rich): a case report.

Giant cell rich malignant fibrous histiocytoma accounts for 3 -15% of all malignant fibrous histiocytomas. Currently, the nomenclature giant cell malignant fibrous histiocytoma is reserved for undifferentiated pleomorphic sarcomas with prominent osteoclastic giant cells. It is considered to be synonymous with malignant giant cell tumor of soft parts. We report a case of disseminated giant cell malignant fibrous histiocytoma involving the scalp, cervical node, lungs, spine, abdominal wall, base of penis, gluteal cleft, paraspinal region and back. The diagnosis was established after staining for a panel of immunohistochemical markers namely cytokeratin, vimentin, S100, desmin, CD68 and smooth muscle actin. CD68 positivity in tumor cells helped in arriving at the final diagnosis. It is essential to recognize this tumor as a giant cell rich distinct entity and differentiate from other giant cell rich pleomorphic sarcomas since therapeutic and prognostic differences are being appreciated currently.

B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis

Host immune response has been considered as an important disease-modifying factor of periodontitis, however, which immune cell(s) or factor(s) are involved in the destruction of periodontium remains unclear. Previously, we reported that osteoclastogenesis is enhanced by activated B cells but suppressed by activated CD8(+)T cells. We present new data that B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis. Purified murine B cells were activated with anti-IgD mAb, IL-4, and anti-CD40 mAb, in the absence (B(Th2)) or presence of Th1 cytokines, either IL-2 (B(IL-2)) or IFN-gamma (B(IFN-gamma)). Each activated B cell population was co-cultured with RAW264.7 cells in the presence of soluble receptor activator of NF-kappaB ligand (sRANKL), and the effect on osteoclastic differentiation was evaluated. While B(Th2)increased osteoclastogenesis, B(IL-2)and B(IFN-gamma)suppressed it profoundly. To verify the mediating molecule(s), we analyzed cytokine profiles of the activated B cells. Compared to B(Th2), B(IL-2)expressed increased amount of IFN-gamma and B(IFN-gamma)expressed decreased amounts of IL-4, IL-5, and IL-10. IFN-gamma was a key negative regulator of osteoclastic differentiation, and mediated the inhibition by B(IL-2). These results suggest that Th1 cytokines may have new important roles in resistance to periodontitis, acting directly on osteoclasts or indirectly through B cells.

Fibroma de células gigantes: estudo histomorfológico de 21 casos e discussäo dos conceitos atuais / Giant cell fibroma: histomorphological study of the 21 cases and discussion of actual concepts

Foram analisados clínica e histomorfologicamente 21 casos de fibroma de células gigantes, diagnosticados no Serviço de Anatomia Patológica de Departamento de Odontologia da UFRN. Verificou-se maior ocorrência no sexo feminino (71,4 por cento) e de raça branca (66,6 por cento); a gengiva revelou-se o sítio anatômico preferencial. Microscopicamente, estas lesöes foram caracterizadas por proliferaçäo de células estreladas ou angulares volumosas, muitas delas exibindo aspecto dendrítico, ocasionalmente, contendo numerosos núcleos, e imersas em um tecido conjuntivo fibroso frouxamente arranjado. Também fotam discutidos alguns conceitos atuais acerca da histogênese desta patologia

Clinical, biochemical and cytomorphological observations in juvenile chronic lymphocytic thyroiditis.

OBJECTIVE: To determine the clinical, biochemical, ultrasonological and cytomorphological features in goitrous juvenile chronic lymphocytic thyroiditis(CLT). SETTING: Tertiary referral center for thyroid disorders. SUBJECTS: A total of 455 children were evaluated for goiter. Of these 122 children had features of CLT in FNAC and were further studied. METHODS: All subjects were subjected to detailed clinical examination. The thyroid functional status was assessed by estimation of serum concentration of thyroid hormones (thyroxine and triiodothyronine) and thyrotropin (TSH). In addition the antithyroid antibody titers were measured. Ultrasonological and cytomorphological characteristics in these patients were also evaluated. RESULTS: The mean age at presentation was 12.5 years (SD 3.93). The male: female ratio was 1:7.7. Thyroid functional status as assessed by serum thyroxine and thyrotropin levels revealed, euthyroidism in 67 (54.9%), hypothyroidism in 30 (24.6%), subclinical hypothyroidism in 22 (18%) and hyperthyroidism in 3 (2.5%). Thyroid antimicrosomal antibodies were detected in significant titers in 90 (73.8%) and antithyroglobulin was positive in 71 (58.2%). The positivity of the antimicrosomal and antithyroglobulin antibodies were much higher in subjects with hypothyroidism and was detected in 86.5% and 69.2%, respectively. The mean urinary iodine excretion was 74.1 micrograms/g of creatinine (SD 31.4) indicating mild iodine deficiency. Fine needle aspiration cytoloty (FNAC) revealed features of chronic lymphocytic thyroiditis. Hurthle cell changes was seen in only 12% of the cases. The epithelium was more often hyperplastic and vacuolation of the cytoplasm and peripheral vacuolations were seen frequently. Giant cells and epithelioid cells were seen in many cases. CONCLUSIONS: In any child presenting with firm goiter, a diagnosis of CLT should be excluded. Many subjects with juvenile CLT have biochemical evidence of hypothyroidism but only few symptoms or clinical features.

Células gigantes multinucleadas em citologia aspirativa de tumor filóide de mama / Multinucleated giant cells in aspiration cytology of phyllodes tumor

O diagnóstico citológico de tumor filóide é baseado em um padräo dimórfico caracterizado pela presença de elementos estromais (fragmentos de tecido conjuntivo ou células fusiformes isoladas) e epiteliais. Entretanto, outras neoplasias primárias da mama, nomeadamente o fibroadenoma, adenomiopitelioma e tumor misto, podem igualmente exibir um quadro citológico semelhante. Neste trabalho descrevemos três casos de tumorfilóide onde a presença de células gigantes multinucleadas foi o achado citológico dominante e auxiliou no diagnóstico diferencial com fibroadenoma. Destacamos a importância deste achado e caracterizamos estas células do ponto de vista imunofenotípico como de linhagem histiocítica